Tag: methods (page 1 of 5)

Why the Government Refuses to Turn Against Monsanto

Ready Or Not ... Here We Come! A Message From Archangel Michael/Ashtar Sheran

Dr. Mercola, GuestIn the video below, Funny or Die pokes fun at Monsanto’s “feeding the world” message by highlighting some of the most obvious features of genetically engineered (GE) foods, such as the unnatural crossing of genetic material between plant and animal kingdoms, the use of toxic chemicals and Monsanto’s ever-expanding monopoly.​“I own everything!” Mama Monsanto exclaims, and that’s pretty close to the truth. Monsanto [...]

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Is The CIA Manipulating The Weather?

Derrick Broze, ContributorIn a recent speech, the director of the Central Intelligence Agency discussed the controversial topic of geoengineering, leading some activists to ask whether the agency is actively and deliberately modifying the weather.​In late June, John Brennan, director of the Central Intelligence Agency, spoke at a Council on Foreign Relations meeting about threats to global security. Director Brennan mentioned a number of threats to stability before di [...]

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Are you Communicating with Your Spirit Guide or Cyberspace Agent? Take the Quiz! ~ Greg Giles

A U.S.cyberspace operations center It should be apparent to all by now that agencies within the U.S. Department of Defense and also within the U.S. intelligence community are actively engaged in programs that utilize the synthetic telepathy technolo...

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Greg Giles ~ Beyond Discernment ~ Who are the Authentic Channels? Part 1

I wish to make it clear that the complicity of Freemasons in this mind control program which uses synthetic telepathy, or voice to skull (V2K) technology, is not a theory I am proposing but point of fact, as the group that had been sending me the '...

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Our Fatally Fractured Food Chain

Julian Rose, ContributorThe term ‘food chain’ refers to the steps that constitute the movement of food from its starting point in the field to its end point on the fork. This incorporates processing and ultimate consumption.The food chain operates within a dynamic life cycle. One which expresses the inseparable interconnection between soil, plant, animal and man – and ends back in the soil again. So that if any one element of this cycle is poisoned or weakened, the [...]

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The Aspirin Alternative Your Doctor Never Told You About

Sayer Ji,  Green Med InfoMillions use aspirin daily without realizing its true dangers. The good news is that there is a natural alternative which preliminary research indicates is safer and more effective.Aspirin is taken faithfully by millions every day as a preventive measure against heart attack, often without the user having any awareness of the serious health risks associated with it, some potentially fatal. You can view over 60 adverse effects of aspirin on the GreenMedIn [...]

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Göbekli Tepe: The Burying Of An Ancient Megalithic Site

Dr. Rita Louise, GuestWhy Did Our Ancestors Inter This Ancient Massive Architectural Wonder?Located at the highest point of the Germus range in the southeastern Anatolia region of Turkey is the mysterious site of Göbekli Tepe. Excavations at Göbekli Tepe commenced in 1995 after German archaeologist Klaus Schmidt realized what was thought to be a Byzantine cemetery was actually a prehistoric site. Schmidt quickly unearthed a number of T-shaped pillars, which set th [...]

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Roswell Alien Slides Unveiled: You be the judge

Alien or mummy? A screenshot of one of the 2 slides which have not yet been released to the public. Excerpt from mirror.co.uk Two photographs of a "dead alien" were unveiled at a big money event last night - and immediately dismissed as fake. A s...

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Lab for genetic modification of human embryos just $2,000 away – report


Reuters / Christian Charisius



Reuters

With the right expertise in molecular biology, one could start a basic laboratory to modify human embryos using a genome-editing computer technique all for a couple thousand dollars, according to a new report.

Genetic modification has received heightened scrutiny recently following last week’s announcement that Chinese researchers had, for the first time, successfully edited human embryos’ genomes. 
The team at Sun Yat-Sen University in Guangzhou, China, used CRISPR (clustered regularly interspaced palindromic repeats), a technique that relies on “cellular machinery” used by bacteria in defense against viruses. 

This machinery is copied and altered to create specific gene-editing complexes, which include the wonder enzyme Cas9. The enzyme works its way into the DNA and can be used to alter the molecule from the inside. The combination is attached to an RNA guide that takes the gene-editing complex to its target, telling Cas9 where to operate. 

Use of the CRISPR technique is not necessarily relegated to the likes of cash-flush university research operations, according to a report by Business Insider. 


Geneticist George Church, who runs a top CRISPR research program at the Harvard Medical School, said the technique could be employed with expert knowledge and about half of the money needed to pay for an average annual federal healthcare plan in 2014 -- not to mention access to human embryos. 

"You could conceivably set up a CRISPR lab for $2,000,” he said, according to Business Insider. 

Other top researchers have echoed this sentiment. 

"Any scientist with molecular biology skills and knowledge of how to work with [embryos] is going to be able to do this,” Jennifer Doudna, a biologist at the University of California, Berkeley, recently told MIT Tech Review, which reported that Doudna co-discovered how to edit genetic code using CRISPR in 2012. 

Last week, the Sun Yat-Sen University research team said it attempted to cure a gene defect that causes beta-thalassemia (a genetic blood disorder that could lead to severe anemia, poor growth, skeletal abnormalities and even death) by editing the germ line. For that purpose they used a gene-editing technique based on injecting non-viable embryos with a complex, which consists of a protective DNA element obtained from bacteria and a specific protein. 

"I suspect this week will go down as a pivotal moment in the history of medicine," wrote science journalist Carl Zimmer for National Geographic.


Response to the new research has been mixed. Some experts say the gene editing could help defeat genetic diseases even before birth. Others expressed concern. 

“At present, the potential safety and efficacy issues arising from the use of this technology must be thoroughly investigated and understood before any attempts at human engineering are sanctioned, if ever, for clinical testing,” a group of scientists, including some who had worked to develop CRISPR, warned in Science magazine. 

Meanwhile, the director of the US National Institutes for Health (NIH) said the agency would not fund such editing of human embryo genes. 

“Research using genomic editing technologies can and are being funded by NIH,” Francis Collins said Wednesday. “However, NIH will not fund any use of gene-editing technologies in human embryos. The concept of altering the human germline in embryos for clinical purposes ... has been viewed almost universally as a line that should not be crossed.”

Although the discovery of CRISPR sequences dates back to 1987 – when it was first used to cure bacteria of viruses – its successes in higher animals and humans were only achieved in 2012-13, when scientists achieved a revolution by combining the resulting treatment system with Cas9 for the first time. 


On April 17, the MIT’s Broad Institute announced that has been awarded the first-ever patent for working with the Crisp-Cas9 system. 

The institute’s director, Eric Lander, sees the combination as “an extraordinary, powerful tool. The ability to edit a genome makes it possible to discover the biological mechanisms underlying human biology.”

The system’s advantage over other methods is in that it can also target several genes at the same time, working its way through tens of thousands of so-called 'guide' RNA sequences that lead them to the weapon to its DNA targets. 

Meanwhile, last month in the UK, a healthy baby was born from an embryo screened for genetic diseases, using karyomapping, a breakthrough testing method that allows doctors to identify about 60 debilitating hereditary disorders.

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Extremely distant exoplanet discovered



 



Excerpt from  thespacereporter.com

According to a NASA statement, the agency’s Spitzer Space Telescope has taken part in the discovery of one of the most distant exoplanets yet found. Spitzer observations were combined with data from the Polish Optical Gravitational Lensing Experiment’s Warsaw Telescope, part of the Las Campanas Observatory in Chile. The newly found exoplanet is approximately 13,000 light-years from Earth, and could yield new clues as to the distribution of planets throughout the Milky Way.

The Warsaw Telescope gathers data through the phenomenon known as microlensing, which occurs when a star passes in front of another, more distant star as seen from Earth’s vantage point. The gravity of the nearer star magnifies and intensifies the distant star’s light; any planets orbiting the distant star appear as small disruptions in the magnification. So far, the microlensing methods has identified around 30 exoplanets, the most distant of which is around 25,000 light-years away.

However, the microlensing method cannot always show how far away are the more distant stars and their planets; the distances to about half of the exoplanets found with microlensing cannot be ascertained. Fortunately, Spitzer is able to help. Located 128 million miles from Earth, Spitzer is able to observe a microlensing event at a different time from the Warsaw Telescope, a method called parallax. In the case of the newly discovered exoplanet, the microlensing event was longer than norman, lasting 150 days. 
Spitzer observed the event 20 days earlier than Warsaw. This time delay allowed the distance to the newly found planet to be calculated. With the distance, the planet’s mass, approximately half that of Jupiter, also was determined.

“We’ve mainly explored our own solar neighborhood so far,” said Sebastiano Calchi Novati of NASA’s Exoplanet Science Institute at the California Institute of Technology. “Now we can use these single lenses to do statistics on planets as a whole and learn about their distribution in the galaxy.”

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Cleaning Up Old Satellites And Space Junk Could Soon Be Done With Giant Fishing Nets

Excerpt from techtimes.com Spacecraft are all shiny and new when they leave Earth but being up in space can do a number on them, resulting into bits and pieces of junk flying around. The European Space Agency's Clean Space initiative is looking to l...

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How Quantum Physics will change your life and amaze the world!

 Excerpt from educatinghumanity.com "Anyone not shocked by quantum mechanics has not yet understood it."Niels Bohr10 Ways Quantum Physics Will Change the WorldEver want to have a "life do over", teleport, time travel, have your computer wor...

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Biologists fear DNA editing procedure can alter human DNA




Excerpt from themarketbusiness.com

A group of biologists was alarmed with the use a new genome-editing technique to modify human DNA in a way that it can become hereditary.
The biologists worry that the new technique is so effective and easy to use that some physicians may push ahead with it before its safety can be weigh up. They also want the public to understand the ethical issues surrounding the technique, which could be used to cure genetic diseases, but also to enhance qualities like beauty or intelligence. The latter is a path that many ethicists believe should never be taken.


“You could exert control over human heredity with this technique, and that is why we are raising the issue,” said David Baltimore, a former president of the California Institute of Technology and a member of the group whose paper on the topic was published in the journal Science.

Ethicists have been concerned for decades about the dangers of altering the human germ line — meaning to make changes to human sperm, eggs or embryos that will last through the life of the individual and be passed on to future generations. Until now, these worries have been theoretical. But a technique invented in 2012 makes it possible to edit the genome precisely and with much greater ease. The technique has already been used to edit the genomes of mice, rats and monkeys, and few doubt that it would work the same way in people.

The new genome-editing technique holds the power to repair or enhance any human gene. “It raises the most fundamental of issues about how we are going to view our humanity in the future and whether we are going to take the dramatic step of modifying our own germline and in a sense take control of our genetic destiny, which raises enormous peril for humanity,” said George Daley, a stem cell expert at Boston Children’s Hospital and a member of the group.

The biologists writing in Science support continuing laboratory research with the technique, and few if any scientists believe it is ready for clinical use. Any such use is tightly regulated in the United States and Europe. American scientists, for instance, would have to present a plan to treat genetic diseases in the human germline to the Food and Drug Administration.

The paper’s authors, however, are concerned about countries that have less regulation in science. They urge that “scientists should avoid even attempting, in lax jurisdictions, germ line genome modification for clinical application in humans” until the full implications “are discussed among scientific and governmental organizations.”

Though such a moratorium would not be legally enforceable and might seem unlikely to exert global sway, there is a precedent. In 1975, scientists worldwide were asked to refrain from using a method for manipulating genes, the recombinant DNA technique, until rules had been established.

“We asked at that time that nobody do certain experiments, and in fact nobody did, to my knowledge,” said Baltimore, who was a member of the 1975 group. “So there is a moral authority you can assert from the U.S., and that is what we hope to do.”

Recombinant DNA was the first in a series of ever-improving steps for manipulating genetic material. The chief problem has always been one of accuracy, of editing the DNA at precisely the intended site, since any off-target change could be lethal. Two recent methods, known as zinc fingers and TAL effectors, came close to the goal of accurate genome editing, but both are hard to use. The new genome-editing approach was invented by Jennifer Doudna of the University of California, Berkeley, and Emmanuelle Charpentier of Umea University in Sweden.

Their method, known by the acronym Crispr-Cas9, co-opts the natural immune system with which bacteria remember the DNA of the viruses that attack them so they are ready the next time those same invaders appear. Researchers can simply prime the defense system with a guide sequence of their choice and it will then destroy the matching DNA sequence in any genome presented to it. Doudna is the lead author of the Science article calling for control of the technique and organized the meeting at which the statement was developed.

Though highly efficient, the technique occasionally cuts the genome at unintended sites. The issue of how much mistargeting could be tolerated in a clinical setting is one that Doudna’s group wants to see thoroughly explored before any human genome is edited.

Scientists also say that replacing a defective gene with a normal one may seem entirely harmless but perhaps would not be.
“We worry about people making changes without the knowledge of what those changes mean in terms of the overall genome,” Baltimore said. “I personally think we are just not smart enough — and won’t be for a very long time — to feel comfortable about the consequences of changing heredity, even in a single individual.”
Many ethicists have accepted the idea of gene therapy, changes that die with the patient, but draw a clear line at altering the germline, since these will extend to future generations. The British Parliament in February approved the transfer of mitochondria, small DNA-containing organelles, to human eggs whose own mitochondria are defective. But that technique is less far-reaching because no genes are edited.

There are two broad schools of thought on modifying the human germline, said R. Alta Charo, a bioethicist at the University of Wisconsin and a member of the Doudna group. One is pragmatic and seeks to balance benefit and risk. The other “sets up inherent limits on how much humankind should alter nature,” she said. 
Some Christian doctrines oppose the idea of playing God, whereas in Judaism and Islam there is the notion “that humankind is supposed to improve the world.” She described herself as more of a pragmatist, saying, “I would try to regulate such things rather than shut a new technology down at its beginning.”

Other scientists agree with the Doudna group’s message.
“It is very clear that people will try to do gene editing in humans,” said Rudolf Jaenisch, a stem cell biologist at the Whitehead Institute in Cambridge, Massachusetts, who was not a member of the Doudna group. “This paper calls for a moratorium on any clinical application, which I believe is the right thing to do.”
Writing in Nature last week, Edward Lanphier and other scientists involved in developing the rival zinc finger technique for genome editing also called for a moratorium on human germline modification, saying that use of current technologies would be “dangerous and ethically unacceptable.”

The International Society for Stem Cell Research said Thursday that it supported the proposed moratorium.

The Doudna group calls for public discussion but is also working to develop some more formal process, such as an international meeting convened by the National Academy of Sciences, to establish guidelines for human use of the genome-editing technique.

“We need some principled agreement that we want to enhance humans in this way or we don’t,” Jaenisch said. “You have to have this discussion because people are gearing up to do this.”

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