Tag: method (page 1 of 12)

What You’ll Never Read About Virus-Research Fraud

Jon Rappoport, GuestThe Rabbit HoleThere are very few investigators on the planet who are interested in this subject. I am one of them. There is a reason why.In many articles, I’ve written about the shocking lack of logic in the curriculum of advanced centers of learning. When I attended college, I was fortunate to have a professor who taught logic, and taught it in a way that appealed to the minds of his students. In other words, for those of us who cared, we could not only ab [...]

View Article Here Read More

Is The CIA Manipulating The Weather?

Derrick Broze, ContributorIn a recent speech, the director of the Central Intelligence Agency discussed the controversial topic of geoengineering, leading some activists to ask whether the agency is actively and deliberately modifying the weather.​In late June, John Brennan, director of the Central Intelligence Agency, spoke at a Council on Foreign Relations meeting about threats to global security. Director Brennan mentioned a number of threats to stability before di [...]

View Article Here Read More

Are you Communicating with Your Spirit Guide or Cyberspace Agent? Take the Quiz! ~ Greg Giles

A U.S.cyberspace operations center It should be apparent to all by now that agencies within the U.S. Department of Defense and also within the U.S. intelligence community are actively engaged in programs that utilize the synthetic telepathy technolo...

View Article Here Read More

Greg Giles ~ The Hidden Agenda of the Galactic Federation Show ~ Part 1

  War in Ukraine   A quick word on Conspiracies: (Thanks to threeworldwars.com) There are three ways of exposing a Conspiracy: 1. One is for any of the participants in the conspiracy to break...

View Article Here Read More

The Nature of Conspiracy & How it is Exposed

 Conspiracy: an evil, unlawful, treacherous, or surreptitious plan formulated in secret by two or more persons. (dictionary.com)  While I near completion on my next blog entry entitled, The Hidden Agenda of the Galactic Federation Progra...

View Article Here Read More

Roll Up Your Sleeves Folks: 271 New Vaccines in Big Pharma’s Pipeline

Gary Kohls, Green Med InfoAs of 2013, Big Pharma has had plans for the development of 271 new vaccines covering an array of diseases.  Into Whose Bodies Will They be Injected?“No vaccine manufacturer shall be liable…for damages arising from a vaccine-related injury or death.” – President Ronald Wilson Reagan, as he signed The National Childhood Vaccine Injury Act (NCVIA) of 1986, absolving drug companies from [...]

View Article Here Read More

How to make oxygen on Mars: Nasa plans to use oxygen cocktail to support human colony

An Indiana firm is developing a method to make oxygen on Mars for Nasa. Bacteria and algae will use Martian soil as fuel to pump out oxygen. Mars could be scattered with biodomes containing the organisms. And future astronauts could use this oxygen to survive on the surface (artist's illustration shown)
An Indiana firm is developing a method to make oxygen on Mars for Nasa. Bacteria and algae will use Martian soil as fuel to pump out oxygen. Mars could be scattered with biodomes containing the organisms. And future astronauts could use this oxygen to survive on the surface (artist's illustration shown)

Excerpt from dailymail.co.uk
  • An Indiana firm is developing a method to make oxygen on Mars for Nasa
  • Bacteria and algae will use Martian soil as fuel to pump out oxygen
  • Mars could be scattered with biodomes containing the organisms
  • And future astronauts could use this oxygen to survive on the surface

If humans land on Mars in the 2030s as planned, one thing that will be essential to their survival will be self-sufficiency, as they won’t be able to take too much cargo with them.

With this in mind Nasa is testing whether oxygen can be created from Martian soil, without having to carry it all the way from Earth.

The innovative method would see bacteria or algae use the soil as fuel, pumping out usable oxygen in the process for astronauts on the surface.

Nasa has been working with Techshot Inc of Greenville, Indiana to develop this method in a so-called ‘Mars room’, which mimics the conditions on the red planet.
It is able to simulate the atmospheric pressure on the planet, in addition to the day-night temperature changes and the solar radiation that hits the surface.

In experiments, certain organisms were capable of producing oxygen from Martian soil - known as regolith - and they also removed nitrogen from it. 

‘This is a possible way to support a human mission to Mars, producing oxygen without having to send heavy gas canisters,’ said Eugene Boland, chief scientist at Techshot.

‘Let’s send microbes and let them do the heavy-lifting for us.’
The research is part of the Nasa Innovative Advanced Concepts (NIAC) Programme.

It’s envisioned that biodomes could be scattered across the surface to produce the oxygen needed for humans to survive.

The oxygen produced could also be stored for later use.

But while experiments on Earth are all well and good, the scientists want to test their method actually on Mars in the near future.

The 'Mars room', shown, is able to simulate the atmospheric pressure on the planet, in addition to the day-night temperature changes and the solar radiation that hits the surface. In experiments certain organisms were capable of producing oxygen from Martian soil inside the laboratory
+3
The 'Mars room', shown, is able to simulate the atmospheric pressure on the planet, in addition to the day-night temperature changes and the solar radiation that hits the surface. In experiments certain organisms were capable of producing oxygen from Martian soil inside the laboratory

When humans land on Mars in the future (artist's illustration of the landing shown), they will need to be as self-sufficient as possible
When humans land on Mars in the future (artist's illustration of the landing shown left), they will need to be as self-sufficient as possible.




To do so, an upcoming rover - such as the 2020 Mars rover - could carry small container-like devices with Earth organisms inside.

The containers would be buried a few inches underground in certain locations, to see how successful they are at producing oxygen.

Sensors inside the container would detect how much oxygen was made, and report the findings back to a satellite in Mars orbit.

The scientists note that the container would be sealed tightly, to prevent the organisms being exposed to - and possibly contaminating - the Martian surface.

But if proven successful, future explorers on Mars may use multiple biodomes like this to produce the oxygen they need to survive.


View Article Here Read More

CRISPR-Cas9 modifies your DNA, under legal fire ~ Video




Excerpt from slashgear.com

A revolutionary method of editing the human genome has this week become the subject of a patent war. Back in April of 2014, patents were awarded by the USPTO (United States Patent and Trademark Office) to the Broad Institutes’ Dr. Feng Zhang, MIT, and Harvard to develop the technology behind "CRISPR-Cas9". 
This April, the UC Board of Regents’ legal team spoke with the USPTO about reconsidering their action, suggesting they award the patent to the inventor of the original method, UC Berkeley’s Jennifer Doudna. One way or another, this radical DNA modifier must be made. 

We need X-Men, after all.


The following video will explain what this genome-editing piece of technological breakthrough is all about. CRISPR-Cas9 is what it's called, and getting in to your body to make changes on a DNA level is what it's going for.

CRISPR-Cas9 works as a tiny scissors.

Utilizing the natural bacterial-level protective system used by your body to fight infections, CRISPR-Cas9 replicates the sequences of target DNA strands and latches on.

It's the connector that CRISPR-Cas9 makes real, and really programmable.

Your body provides the Cas9.* The Cas9 is the nuclease enzyme that cuts DNA strands.

*Correction - BACTERIA have Cas9, not our human selves. As helpful commenter "John" put so eloquently, "So far it has only been found in bacterial cells, and that's one of the things that makes it so amazing--a relatively simple molecular system that replicates many of the functions of our elaborate adaptive immune response, all in a single prokaryotic cell!"

Screen Shot 2015-05-15 at 10.11.42 AM
ABOVE: Image comes via Nature; Addgene, By Jonathan Corum, via NYT.

When a DNA sequence is cut, it may attempt to re-form. In doing so, it can create mutations.

But that's a discussion for another day.

fx1

Genetically altered twin monkeys have been made using the CRISPR-Cas9 method. As you'll see in DOI: http://dx.doi.org/10.1016/j.cell.2014.01.027 in Cell Symposia "Generation of Gene-Modified Cynomolgus Monkey via Cas9/RNA-Mediated Gene Targeting in One-Cell Embryos", these monkeys are living large on gene-altered action.

12DOUDNAjp-articleLarge

Today what's important is that two groups are fighting for the patents involved in CRISPR-Cas9.

A paper published online June 28 2012 by Science authored by Martin Jinek, Krzysztof Chylinski, Ines Fonfara, Michael Hauer, Jennifer A. Doudna, and Emmanuelle Charpentier describes the method: "A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity."

You can find this paper under code DOI: 10.1126/science.1225829.

Dr. Zhang suggests he demonstrated the CRISPR genome editing method before the 2012 paper was published by Dr. Doudna and Dr. Charpentier and crew. Dr. Doudna and Dr. Charpentier and crew suggest say Dr. Zhang's notebook (used as proof for patents) does not prove genome editing before the 2012 paper was published.

Can't we all just get along? Think of the monkeys!

Click to zoom

View Article Here Read More

6 Ways to Overcome a Soul-Crushing Life Challenge




Excerpt from huffingtonpost.com

It was never in your life plan, certainly never predicted in your high school yearbook.
And yet, here you are. You've gone through a soul-sucking life experience and are suffering from the collateral consequences. Uncertainty, fear and disbelief rule the day. You keep waiting to wake up and find out this was all a bad dream.
The problem is that wishing, wanting and waiting don't help. Whether you're still in the midst of the storm or idling in the aftermath, the truth is that you have to reach down and make the decision that although you may have had no control over what happened to you, you do have control over how you respond and move forward. These six tips will help start you on that journey:
1. Don't Compare Your Blooper Reel to Other's Highlight Reel
At times it may seem like the grass is greener on the other side of the fence. Social media exacerbates this perception because people tend to show only their green patch of lawn and not their backyard full of weeds!

Wouldn't it be refreshing to see someone's perfect vacation pictures captioned: "Don't know how we're really going to pay for this; We're up to our ears in debt! The kids got carsick and puked in the rental car, and Jack and I haven't had sex for weeks! Wish you were here!"
The grass isn't always greener. Everyone has something in life they wish they could undo, redo or erase. They just don't post it on Facebook.
2. Realize That Sometimes You Have No Control Over What Happens to You
Like the saying goes, life is what happens to you when you are making other plans. I truly believe that things happen for you rather than to you to nudge you into growth. When something unexpected happens, ask yourself "What's the lesson here?"

3. Surrender to Your Situation
Surrendering doesn't mean giving in; it simply means you stop fighting the fact that the situation happened. Accept the fact that it occurred, that it sucks, and that yes, it probably was unfair and undeserved.

When you continually try to fight against a situation, it's like trying to swim against a rip current. You can fight it and end up exhausted and pulled out to sea, or you can accept that it is done, swim parallel to it and overcome it. You cannot change what has already occurred but you can change how you respond to it. This is the tipping point to taking your power back.
4. Understand That Your Coping Mechanisms May Be Holding You Hostage
It is natural to feel disbelief, anger and sadness, and to want to blame others for what you are going through. These coping mechanisms are designed to help you deal with the situation at hand. They are also a defense mechanism, a way to push back on the reality of the situation.

The problem is, when you get stuck defending, denying, and blaming, you form an endless loop of negative thoughts that won't stop spinning in your head. The part of your brain that is controlling the loop is your ego. When you learn to harness your ego, you can transform the way you think and move past these self-destructive thoughts.
5. Harness Your Ego
Your ego is part of your consciousness, and it competes with your higher self, or spirit, for control of your thoughts. Your ego is fear-based and your higher self is love-based. The two cannot coexist because the higher self simply does not recognize fear. Think of the ego as the darkness and the higher self as the light switch; once the light goes on darkness cannot exist.

The ego thrives on fear and separation in order to control your thoughts. It causes you to think you need to be better because you're not good enough or are lacking in some way. The egoic brain creates this fear of inferiority and you react by putting others down as a way to raise your sense of self-worth up.
You can recognize your ego at work when you are critical or judgmental of others, when you take on the role of victim, or when you blame others rather than looking inward. When you feel self-important, when you feel the need to be right, and when anger, jealousy, and self-importance take center stage, that's your ego, and it isn't helping you. It creates a false sense of self.
Once you are aware that your ego is talking, you have begun the process of winning the mind chatter war in your head. Your awareness helps you realize that you no longer have to react to the fear it is creating. Your thoughts are not you but are of the ego. Remember that your ego and your higher self cannot co-exist; When you recognize the ego it has to take a back seat to your higher self. You then can move above these thoughts and shift your perspective from negative thoughts to ones that serve you positively.
6. Create Calm and Gratitude
The ego loves for you to focus on your past, on what you lost. What if you shift the way you look at your situation and focus on what you gained as a result?

What did you learn as a result of the trial? Are you more compassionate, less judgmental? Is your house calmer or cleaner? Did you start taking better care of yourself emotionally or physically? Are you finally putting yourself first?
Focusing on what you are grateful for instead of what you lost is a mindset that creates a calmer, happier you. And that is something to be grateful for!
Tired of feeling like you'll never be happy again? Mary Holloway empowers women to reach down and find their inner warrior. Mary is a sought after speaker, resilience coach, and most importantly, a survivor of soul crushing life experiences. She is the founder of Resilience Café and the creator of the Boom Bounce Wow Resilience Method. Mary believes that every woman has an incredible warrior within her that can help her take back her life and emerge better, stronger, happier, and surprisingly thankful for the experience. She knows that women can choose not to be defined by their situation but rather by how they respond to it.
Follow Mary at ResilienceCafe.com...

View Article Here Read More

Lab for genetic modification of human embryos just $2,000 away – report


Reuters / Christian Charisius



Reuters

With the right expertise in molecular biology, one could start a basic laboratory to modify human embryos using a genome-editing computer technique all for a couple thousand dollars, according to a new report.

Genetic modification has received heightened scrutiny recently following last week’s announcement that Chinese researchers had, for the first time, successfully edited human embryos’ genomes. 
The team at Sun Yat-Sen University in Guangzhou, China, used CRISPR (clustered regularly interspaced palindromic repeats), a technique that relies on “cellular machinery” used by bacteria in defense against viruses. 

This machinery is copied and altered to create specific gene-editing complexes, which include the wonder enzyme Cas9. The enzyme works its way into the DNA and can be used to alter the molecule from the inside. The combination is attached to an RNA guide that takes the gene-editing complex to its target, telling Cas9 where to operate. 

Use of the CRISPR technique is not necessarily relegated to the likes of cash-flush university research operations, according to a report by Business Insider. 


Geneticist George Church, who runs a top CRISPR research program at the Harvard Medical School, said the technique could be employed with expert knowledge and about half of the money needed to pay for an average annual federal healthcare plan in 2014 -- not to mention access to human embryos. 

"You could conceivably set up a CRISPR lab for $2,000,” he said, according to Business Insider. 

Other top researchers have echoed this sentiment. 

"Any scientist with molecular biology skills and knowledge of how to work with [embryos] is going to be able to do this,” Jennifer Doudna, a biologist at the University of California, Berkeley, recently told MIT Tech Review, which reported that Doudna co-discovered how to edit genetic code using CRISPR in 2012. 

Last week, the Sun Yat-Sen University research team said it attempted to cure a gene defect that causes beta-thalassemia (a genetic blood disorder that could lead to severe anemia, poor growth, skeletal abnormalities and even death) by editing the germ line. For that purpose they used a gene-editing technique based on injecting non-viable embryos with a complex, which consists of a protective DNA element obtained from bacteria and a specific protein. 

"I suspect this week will go down as a pivotal moment in the history of medicine," wrote science journalist Carl Zimmer for National Geographic.


Response to the new research has been mixed. Some experts say the gene editing could help defeat genetic diseases even before birth. Others expressed concern. 

“At present, the potential safety and efficacy issues arising from the use of this technology must be thoroughly investigated and understood before any attempts at human engineering are sanctioned, if ever, for clinical testing,” a group of scientists, including some who had worked to develop CRISPR, warned in Science magazine. 

Meanwhile, the director of the US National Institutes for Health (NIH) said the agency would not fund such editing of human embryo genes. 

“Research using genomic editing technologies can and are being funded by NIH,” Francis Collins said Wednesday. “However, NIH will not fund any use of gene-editing technologies in human embryos. The concept of altering the human germline in embryos for clinical purposes ... has been viewed almost universally as a line that should not be crossed.”

Although the discovery of CRISPR sequences dates back to 1987 – when it was first used to cure bacteria of viruses – its successes in higher animals and humans were only achieved in 2012-13, when scientists achieved a revolution by combining the resulting treatment system with Cas9 for the first time. 


On April 17, the MIT’s Broad Institute announced that has been awarded the first-ever patent for working with the Crisp-Cas9 system. 

The institute’s director, Eric Lander, sees the combination as “an extraordinary, powerful tool. The ability to edit a genome makes it possible to discover the biological mechanisms underlying human biology.”

The system’s advantage over other methods is in that it can also target several genes at the same time, working its way through tens of thousands of so-called 'guide' RNA sequences that lead them to the weapon to its DNA targets. 

Meanwhile, last month in the UK, a healthy baby was born from an embryo screened for genetic diseases, using karyomapping, a breakthrough testing method that allows doctors to identify about 60 debilitating hereditary disorders.

View Article Here Read More

Extremely distant exoplanet discovered



 



Excerpt from  thespacereporter.com

According to a NASA statement, the agency’s Spitzer Space Telescope has taken part in the discovery of one of the most distant exoplanets yet found. Spitzer observations were combined with data from the Polish Optical Gravitational Lensing Experiment’s Warsaw Telescope, part of the Las Campanas Observatory in Chile. The newly found exoplanet is approximately 13,000 light-years from Earth, and could yield new clues as to the distribution of planets throughout the Milky Way.

The Warsaw Telescope gathers data through the phenomenon known as microlensing, which occurs when a star passes in front of another, more distant star as seen from Earth’s vantage point. The gravity of the nearer star magnifies and intensifies the distant star’s light; any planets orbiting the distant star appear as small disruptions in the magnification. So far, the microlensing methods has identified around 30 exoplanets, the most distant of which is around 25,000 light-years away.

However, the microlensing method cannot always show how far away are the more distant stars and their planets; the distances to about half of the exoplanets found with microlensing cannot be ascertained. Fortunately, Spitzer is able to help. Located 128 million miles from Earth, Spitzer is able to observe a microlensing event at a different time from the Warsaw Telescope, a method called parallax. In the case of the newly discovered exoplanet, the microlensing event was longer than norman, lasting 150 days. 
Spitzer observed the event 20 days earlier than Warsaw. This time delay allowed the distance to the newly found planet to be calculated. With the distance, the planet’s mass, approximately half that of Jupiter, also was determined.

“We’ve mainly explored our own solar neighborhood so far,” said Sebastiano Calchi Novati of NASA’s Exoplanet Science Institute at the California Institute of Technology. “Now we can use these single lenses to do statistics on planets as a whole and learn about their distribution in the galaxy.”

View Article Here Read More

Boeing to Create a Star Wars-like Forcefield (Yes, an actual forcefield) ~ Video

Excerpt from usatoday.comStar Wars jokes aside, Boeing actually just patented a force field that would use energy to deflect explosions. For real; it's called, "method and system for shockwave attenuation via electromagnetic arc."According to the pat...

View Article Here Read More

Cleaning Up Old Satellites And Space Junk Could Soon Be Done With Giant Fishing Nets

Excerpt from techtimes.com Spacecraft are all shiny and new when they leave Earth but being up in space can do a number on them, resulting into bits and pieces of junk flying around. The European Space Agency's Clean Space initiative is looking to l...

View Article Here Read More
Older posts

Creative Commons License
This work is licensed under a
Creative Commons Attribution 4.0
International License
.
unless otherwise marked.

Terms of Use | Privacy Policy



Up ↑